Systems Biology - Organisms - Shewanella oneidensis

From Keaslingwiki

Shewanella oneidensis MR-1 is able to utilize many carbon sources and reduce a variety of electron acceptors. The versatile respiration ability of S. oneidensis MR-1 may be used to generate electricity from many substrates or used in bioremediation applications involving a variety of toxic metals. The goal of our study is to elucidate the aerobic and anaerobic pathways in Shewanella strains. We grew Shewanella oneidensis MR-1 in the presence of 13C lactate under various conditions. The resulting 13C labeling patterns of key metabolites, including several amino acids, pyruvate, and succinate, were determined using GC/MS, NMR or FT-ICR. These labeling patterns permitted the identification of the active routes of carbon utilization in the main metabolic pathways of S. oneidensis MR-1. Interpreted in the light of reported enzymatic activities and genome annotation information, we use isotopomer models to calculate the carbon fluxes through the central pathways in MR-1. The techniques we have developed for flux analysis can also be applied for flux analysis in other microorganisms.