Christopher Petzold

Christopher J. Petzold

Current Research Projects

• Proteomics

Research Summary

Mass spectrometry is a useful tool to identify and quantify biological molecules in complex mixtures. By using isotopic-labeling techniques (e.g., iTRAQ) coupled with tandem LC/MS the bacterial proteome can be analyzed and compared under a variety of growth and environmental conditions. The relative protein levels present in the bacteria prior to and after induction of the amorphadiene pathway yield insight into the mechanisms E. coli uses to respond to the drain on resources and important metabolite pools. A thorough understanding of the cellular processes leading to these deleterious effects will permit modification of the production system (e.g., host, media, growth conditions) to minimize or eliminate the harmful components.

Background

Education

• Postdoctorate, Chemistry, University of California (Berkeley and Davis), 2002-2005
• Ph.D. Analytical Chemistry, Purdue University, 2002 (Kenttämaa Group)
• B.S. Chemistry, Taylor University, 1997
• B.A. Physics, Taylor University, 1997

Publications

• Pérez, J.; Petzold, C. J.; Watkins, M. A.; Vaughn, W. E.; Kenttämaa, H. I. “Laser Desorption in Transmission Geometry Inside a Fourier-Transform Ion Cyclotron Resonance Mass Spectrometer”, J. Am. Soc. Mass Spectrom. 1999, 10(11), 1105-1110.
• Burden, S. L.; Petzold, C. J. “Antacids Revisited with Modern Chemical Instruments: GCMS, AAS, and CCT”, J. Chem. Ed. 1999, 76(11), 1544-1547.
• Pérez, J.; Ramírez-Arizmendi, L. E.; Petzold, C. J.; Guler, L. P.; Nelson, E. D.; Kenttämaa, H. I. “Laser-Induced Acoustic Desorption/Chemical Ionization in Fourier-transform Ion Cyclotron Resonance Mass Spectrometry”, Int. J. Mass Spectrom. 2000, 198, 173-188.
• Price, J. M.; Petzold, C. J.; Byrd, H. C. M.; Kenttämaa, H. I. “An Examination of Barriered and Barrierless Hydrogen Atom Abstractions by Organic Radical Cations: the Cytosine Radical Cation”, Int. J. Mass Spectrom. 2001, 212, 455-466.
• Petzold, C. J.; Ramírez-Arizmendi, L. E.; Heidbrink, J. L.; Pérez, J.; Kenttämaa, H. I. “Gas-phase Reactions of Charged Phenyl Radicals with Neutral Biomolecules Evaporated by Laser-induced Acoustic Desorption”, J. Am. Soc. Mass Spectrom. 2002, 13(2), 192-194.
• Petzold, C. J.; Nelson, E. D.; Lardin, H. A.; Kenttämaa, H. I. “Charge-Site Effects on the Radical Reactions of Distonic Ions”, J. Phys. Chem. A 2002, 106(42), 9767-9775.
• Amegayibor, F. S.; Nash, J. J.; Lee, A. S.; Thoen, J.; Petzold, C. J.; Kenttämaa, H. I. “Chemical Properties of a para-Benzyne”, J. Am. Chem. Soc. 2002, 124(41), 12066-12067.
• Gao, H.; Petzold, C. J.; Leavell, M. D.; Leary, J. A. “Ion/Molecule Reaction for Quantification of Phosphorylated Diastereomers” J. Am. Soc. Mass Spectrom. 2003, 14, 916-924.
• Petzold, C. J.; Leavell, M. D.; Leary, J. A. “Screening, and Identification of Acidic Carbohydrates in Bovine Colostrum by using Ion/Molecule Reactions and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Specificity toward Phosphorylated Complexes”, Anal. Chem. 2004, 76(1), 203-210.
• Mougous, J. D.; Petzold, C. J.; Senaratne, R. H.; Lee, D. H.; Akey, D. L.; Riley, L. W.; Leary, J. A.; Berger, J. M.; Bertozzi, C. A. “Identification, Function and Structure of the Sulfotransferase that Initiates Sulfolipid-1 Biosynthesis in Mycobacterium tuberculosis”, Nat. Struct. Biol. 2004, 11(8), 721-729.
• Petzold, C. J.; Stanton L. H.; Leary, J. A. “Structural Characterization of Mild Acid Hydrolyzed Lipoarabinomannans from Mycobacterium tuberculosis and Mycobacterium smegmatis by ESI Mass Spectrometry”, J. Am. Soc. Mass Spectrom. 2005, 16(7), 1109-1116.
• Liu, J. A.; Petzold, C. J.; Ramírez-Arizmendi, L. E.; Pérez, J.; Kenttämaa, H. I. "Phenyl radicals react with dinucleoside phosphates by addition to purine bases and H-atom abstraction from a sugar moiety", J. Am. Chem. Soc. 2005, 127(37), 12758-12759.
• Mougous, J. D.; Senaratne, R. H. Petzold, C. J.; Jain, M.; Lee, D. H.; Schelle, M. A.; Leavell, M. D.; Cox, J. S.; Leary, J. A.; Riley, L. E.; Bertozzi, C. R. “A Novel Sulfated Metabolite Produced by stf3 Negatively Regulates the Virulence of M. tuberculosis”, Proc. Natl. Acad. Sci. U S A. 2006, 103(11), 4258-4263.
• Shea, R. C.; Petzold, C. J.; Campbell, J. L.; Li, S.; Aaserud, D. J.; Kenttamaa, H. I. "Characterization of laser-induced acoustic desorption coupled with a fourier transform ion cyclotron resonance mass spectrometer", Anal. Chem. 2006, 78(17), 6133-6139.
• Shea, R. C.; Petzold, C. J.; Liu J.; Kenttämaa, H. I. "Experimental Investigations of the Internal Energy of Molecules Evaporated via Laser-Induced Acoustic Desorption into a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer", Anal. Chem. 2007, 79(5), 1825-1832.
• Jain, M.; Petzold, C. J.; Schelle, M. W.; Leavell, M. D.; Mougous, J. D.; Bertozzi, C. R.; Leary, J. A.; Cox, J. S. "Lipidomics reveals control of Mycobacterium tuberculosis virulence lipids via metabolic coupling", Proc. Natl. Acad. Sci. U S A. 2007, 104(12), 5133-5138.
• Kumar, P.; Schelle, M. W.; Jain, M.; Lin, F. L.; Petzold, C. J.; Leavell, M. D.; Leary, J. A.; Cox, J. S.; Bertozzi, C. R. “PapA1 and PapA2 are acyltransferases essential for the biosynthesis of the Mycobacterium tuberculosis virulence factor Sulfolipid-1”, Proc. Natl. Acad. Sci. U S A. 2007, 104(27), 11221-11226.
• Mahendra, S.; Petzold, C. J.; Baidoo, E. E.; Keasling, J. D.; Alvarez-Cohen, L. "Identification of the Intermediates of in Vivo Oxidation of 1,4-Dioxane by Monooxygenase-Containing Bacteria", Environ. Sci. Technol. 2007, 41(21), 7330-7336.
• Shui, W.; Sheu, L.; Liu, J.; Smart, B.; Petzold, C. J.; Pitcher, A.; Hsieh, Y.; Keasling, J. D.; Bertozzi, C. R. “Membrane Proteomics of Phagosomes Suggests a Connection to Autophagy”, Proc. Natl. Acad. Sci. U S A. 2008, 105(44), 16952-16957.
• Steen, E. J.; Chan, R.; Prasad, N.; Myers, S.; Petzold, C.J.; Redding, A.; Ouellet, M.; Keasling, J. D. "Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol" Microbial Cell Factories (submitted)

Presentations

• C. J. Petzold, L. Kizer, W. Shui, W. Marner, R. Dahl, D. Garcia, and J. D. Keasling “A Quantitative Proteomic Study of Escherichia coli Expressing an Exogenous Pathway for Amorphadiene Production”, 54st Annual American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics, Seattle, WA 2006. (Poster presentation)