User:Alyssa

Alyssa Redding

Research Project with the Keasling Lab

Understanding Stress Response of Desulfovibrio vulgaris Hildenborough

Research Summary

I use proteomics to evaluate changes in the physiology of the anaerobic bacterium Desulfovibrio vulgaris Hildenborough under a variety of stress conditions. Using the iTRAQ peptide labeling technique, proteins from many different conditions can be compared quantitatively by mass spectrometry. I use QTOF and MALDI mass spectrometry combined with nano-flow HPLC. For more information, you can follow the links to the Environmental Stress Pathway Projectof the Virtual Institute for Microbial Stress and Survival (VIMSS).

Current Projects

I perform proteomics research as part of the Technology Division of JBEI

Background

Education

• B.S. in Chemical Engineering from University of Utah, Salt Lake City, Utah. 2002.
• Ph.D. in Chemical Engineering from University of California, Berkeley, Berkeley, California, 2007.

Publications

• Redding, AM, Mukhopadhyay A, Joyner D, Hazen TC, Keasling JD. Study of Nitrate Stress in Desulfovibrio vulgaris Hildenborough Using iTRAQ Proteomics. Briefings in Functional Genomics and Proteomics. 2006, 5:133-143.
• A. Mukhopadhyay, Z. He, E. J. Alm, A. P. Arkin, E. E. Baidoo, S. C. Borglin, W. Chen, T. C. Hazen, Qiang He, H.-Y. Holman, K. Huang, R. Huang, D. C. Joyner, N. Katz, M. Keller, P. Oeller, A. Redding, J. Sun, J. Wall, J. Wei, Z. Yang , H.-C. Yen, J. Zhou, and J. D. Keasling. 2006 Salt stress in Desulfovibrio vulgaris Hildenborough: An integrated genomics approach. Journal of Bacteriology. 2006, 188:4068-4078.

Presentations

• Redding AM, Mukhopadhyay A, Petzold CJ, Gilman A, Joyner D, Hazen TC, Keasling JD. Stress Response in Desulfovibrio vulgaris Hildenborough: A Proteomics Approach. Poster Presentation at 7th Siena Meeting From Genome to Proteome: Back to the Future, Siena, Italy 2006.
• Redding AM, Keasling JD. A Functional Genomics Study of Stress Response in Desulfovibrio vulgaris. American Institute of Chemical Engineers 2005 Annual Meeting, Cincinnati, OH 2005.
• Redding AM, Mukhopadhyay A, Joyner D, Hazen TC, Keasling JD. Stress Response in Desulfovibrio vulgaris Hildenborough: A Proteomics Approach. Poster presentation at 13th Annual International Meeting on Microbial Genomes. Madison, WI 2005.
• Redding AM, Mukhopadhyay A, Martin VJJ, Keasling JD. Proteomics of Desulfovibrio vulgaris Hildenborough. ASM Conference on Integrating Metabolism and Genomics. Montreal, Canada 2004.