William J. Holtz
Ph.D. Electrical Engineering and Computer Science 2011 UC Berkeley: Engineering Scalable Combinational Logic in Escherichia coli Using Zinc Finger Proteins
My research is focused on engineering control of transcription using zinc finger proteins. With zinc fingers proteins I am designing methods of regulating native operons and creating scalable sets of logic gates. Microbes can sense many environmental conditions and there are a wide variety of outputs they can produce. Currently synthetic biologists connect these inputs to an output with a one-to-one correspondence or a two-to-one configuration in advanced systems. This limitation is mostly due to our lack of large numbers of devices that can operate independently in a single cell. By utilizing zinc-finger proteins and other scalable interaction domains, I aim to make a large library of independent transcription control devices that can be utilized to integrate many input signals into a single output.
D.C. Huang, W.J. Holtz, M.M. Maharbiz. (2012). "A genetic bistable switch utilizing nonlinear protein degradation." Journal of biological engineering, 6(1), 9.
J. Hsia, W.J. Holtz, D.C. Huang, M. Arcak, M.M. Maharbiz. (2012). "A feedback quenched oscillator produces turing patterning with one diffuser." PLoS computational biology, 8(1), e1002331.
T.S. Lee, R.A. Krupa, F. Zhang, M. Hajimorad, W.J. Holtz, N Prasad, S.K. Lee, J.D. Keasling. (2011). "BglBrick vectors and datasheets: a synthetic biology platform for gene expression." Journal of biological engineering, 5(1), 1-14.
- W.J. Holtz and J.D. Keasling, “Engineering Static and Dynamic Control of Synthetic Pathways,” Cell, vol. 140, pp. 19-23, 2010.
- F.J. Zendejas, U. Srinivasan, W.J. Holtz, J.D. Keasling, R.T. Howe, "Microfluidic Generation of Tunable Emulsions for Templated Monodisperse Silica," Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. Transducers '05. The 13th International Conference on , vol.2, no.pp. 1473- 1476, 2005.
- M.M. Maharbiz, W.J. Holtz, R.T. Howe, J.D. Keasling, "Microbioreactor Arrays With Parametric Control for High-Throughput Experimentation," Biotechnology and Bioengineering, vol. 85(4), pg. 376-381, 2004.
- M.M. Maharbiz, W.J. Holtz, S. Sharifzadeh, J.D. Keasling, R.T. Howe, "A Microfabricated Electrochemical Oxygen Generator for High-Density Cell Culture Arrays," Microelectromechanical Systems, Journal of , vol.12, no.5, pp. 590- 599, Oct. 2003.
- M.M. Maharbiz, W.J. Holtz, S. Sharifzadeh, J.D. Keasling and R.T. Howe, "A Microfabricated Electrochemical Oxygen Generator for High-Density Cell Culture Arrays," in Solid-State Sensor, Actuator, and Microsystems Workshop, 2002, pp. 259-264.
- J. Yang, W. Holtz, W. Yang and M. Tue Vo, "An Adaptive Multimodal Interface for Wireless Applications," in Second International Symposium on Wearable Computers, 1998, pp. 172-173.
Intel's Robert N. Noyce Graduate Research Fellowship
Merck Best Poster Award, Biochemical Engineering XIII, 2003