Ph.D. The University of Tsukuba, 2008

M.S.  The University of Tsukuba, 2005

B.S.  The University of Tsukuba, 2003


Office 4460Q
Bench 4550-037B
5885 Hollis St., 4th floor
Emeryville, CA 94608

Current Research

At JBEI, I’m working on producing high-value chemicals by expressing type I modular polyketide synthase genes in Streptomyces strains.


Fujii T, Okuda N, Inoue H, Ishikawa K, Hoshino T., Enhancing cellulase production by overexpression of xylanase regulator protein gene, xlnR, in Talaromyces cellulolyticus cellulase hyperproducing mutant strain.. Biosci. Biotechnol. Biochem. In press.


Fujii T, Inoue H, Ishikawa K., Decreased cellulase and xylanase production in the fungus Talaromyces cellulolyticus by disruption of tacA and tctA genes, encoding putative zinc finger transcriptional factor. Appl. Biochem. Biotechnol. Vol. 175, pp3218-3229, 2015.


Fujii T, Koike K, Yano S, Sawayama S, Inoue H., Draft Genome Sequence of Talaromyces cellulolyticus Y-94, a Source of Lignocellulosic Biomass-Degrading Enzyme. Genome Announcements. Vol. 3 (1), 2015.


Inoue H, Kshishita S, Kumagai A, Kataoka M, Fujii T, Ishikawa K., Contribution of a family 1 carbohydrate-binding module in thermostable glycoside hydrolase 10 xylanase from Talaromyces cellulolyticus toward synergistic enzymatic hydrolysis of lignocellulose. Biotechnol. Biofuels. Vol 8:77, 2015


Kishishita S, Fujii T, Ishikawa K., Heterologous expression of hyperthermophilic cellulases of archaea Pyrococcus sp. by fungus Talaromyces cellulolyticus. J. Ind. Microbiol. Biotechnol. Vol 42, pp137-141, 2015


Fujii T, Inoue H, Ishikawa K., Characterization of the xylanase regulator protein gene, xlnR, in Talaromyces cellulolyticus (formerly known as Acremonium cellulolyticus). Biosci. Biotechnol. Biochem. Vol 78, pp1564-1567, 2014


Fujii T, Hoshino T, Inoue H, Yano S., Taxonomic revision of the cellulose-degrading fungus Acremonium cellulolyticus nomen nudum to Talaromyces (Penicillium) based on phylogenetic analysis. FEMS Microbiol. Letters. Vol 351, pp32-42, 2014


Fujii T, Murakami K, Endo T, Fujimoto S, Minowa T, Matsushika A, Yano S, Sawayama S., Bench Scale Bioethanol Production from Eucalyptus by High Solid Saccharification and Glucose/Xylose Fermentation Method. Bioprocess Biosyst. Eng. Vol 37, pp749-754, 2014


Hayata K, Asada S, Fujii T, Inoue H, Ishikawa K, Sawayama S., Gene targeting by RNAi-mediated knockdown of potent DNA ligase IV homologue in the cellulase-producing fungus Talaromyces cellulolyticus. Appl. Biochem. Biotechnol., Vol 174, pp1697-1704, 2014


Watanabe M, Inoue H, Inoue B, Yoshimi M, Fujii T, Ishikawa K., Xylanase (GH11) from Acremonium cellulolyticus: homologous expression and characterization. AMB Express.Vol. 4:27, 2014.


Asada S, Watanabe S, Fujii T, Inoue H, Ishikawa K, Sawayama S., RNAi knockdown of potent sugar sensor in cellulase-producing fungus Acremonium cellulolyticus. Appl. Biochem. Biotechnol., Vol 172, pp3009-3015, 2014


Kishishita S, Yoshimi M, Fujii T, Taylor LE, Decker SR, Ishikawa K., Cellulose-inducible xylanase Xyl10A from Acremonium cellulolyticus: purification, cloning and homologous expression. Protein Expres. Purif., Vol 94, pp40-45, 2014


Fujii T, Inoue H, Ishikawa K., Enhancing cellulase and hemicellulase production by genetic modification of carbon catabolite repressor gene, creA, in Acremonium cellulolyticus. AMB Express. Vol 3, 73, 2013


Fujii T, Matsushika A, Goshima T, Murakami K, Yano S., Comparison of the Performance of Eight Recombinant Strains of Xylose-Fermenting Saccharomyces cerevisiae As to Bioethnoal Production from Rice Straw Enzymatic Hydrolyzate. Biosci. Biotechnol. Biochem. Vol 77, pp1579-1582, 2013


Inoue H, Fujii T, Yoshimi M, Taylor LE, Decker SR, Kishishita S, Nakabayashi M, Ishikawa K., Construction of a starch-inducible homologous expression system to produce cellulolytic enzymes from Acremonium cellulolyticus. J. Ind. Microbiol. Biotechnol. Vol 40, pp823-830, 2013


Hideno A, Inoue H, Fujii T, Yano S, Tsukahara K, Murakami K, Yunokawa H, Sawayama S., High-coverage gene expression profiling analysis of the cellulase-producing fungus Acremonium cellulolyticus cultured using different carbon sources. Appl. Microbiol. Biotechnol. Vol 97, pp5483-5492, 2013


Fujii T, Iwata K, Murakami K, Yano S, Sawayama S., Isolation of Uracil Auxotrophs of the Fungus Acremonium cellulolyticus and the Development of a Transformation System with the pyrF Gene. Biosci. Biotechnol. Biochem. Vol 76, pp245-249, 2012


Matsushika A, Goshima T, Fujii T, Inoue H, Sawayama S, Yano S., Characterization of non-oxidative transaldolase and transketolase enzymes in the pentose phosphate pathway with regard to xylose utilization by recombinant Saccharomyces cerevisiae. Enzyme. Microb. Technol. Vol 51(1), pp16-25, 2012.


Terabayashi Y, Shimizu M, Kitazume T, Masuo S, Fujii T, Takaya N., Conserved and specific responses to hypoxia in Aspergillus oryzae and Aspergillus nidulans determined by comparative transcriptomics. Appl. Microbiol. Biotechnol. Vol 93, pp305-317, 2012


Fujii T, Yu G, Matsushika A, Kurita A, Yano S, Murakami K, Sawayama S., Ethanol production from xylo-oligosaccharides by xylose-fermenting Saccharomyces cerevisiae expressing β-xylosidase. Biosci. Biotechnol. Biochem. Vol 75, pp1140-1146,2011


Kanna M, Yano S, Inoue H, Fujii T, Sawayama S., Enhancement of β-xylosidase productivity in cellulase producing fungus Acremonium cellulolyticus. AMB Express. Vol 1(1):15, 2011


Sato I, Shimatani K, Fujita K, Abe T, Shimizu M, Fujii T, Hoshino T, Takaya N., Glutathione reductase/glutathione is responsible for cytotoxic elemental sulfur tolerance via polysulfide shuttle in fungi. J. Biol. Chem. Vol 286, pp20283-20291, 2011


Fujii T, Shimizu M, Masuo S, Takaya N., Mechanism of de novo branched-chain amino acid synthesis as an alternative electron sink in hypoxic Aspergillus nidulans cells. Appl. Environ. Microbiol. Vol 76, pp1507-1515, 2010


Fujii T, Murakami K, Sawayama S., Cellulase hyperproducing mutants derived from the fungus Trichoderma reesei QM9414 produced large amounts of cellulase at the enzymatic and transcriptional levels. Biosci. Biotechnol. Biochem. Vol 74, pp419-422. 2010


Masuo S, Terabayashi Y, Shimizu M, Fujii T, Kitazume T, Takaya N., Global gene expression analysis of Aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia. Mol. Genet. Genomics. Vol 284, pp415-424. 2010


Fujii T, Fang X, Inoue H, Murakami K, Sawayama S., Enzymatic hydrolyzing performance of Acremonium cellulolyticus and Trichoderma reesei against three lignocellulosic materials. Biotechnol. Biofuels. Vol 2:24., 2009


Shimizu M, Fujii T, Masuo S, Fujita K, Takaya N., Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions. Proteomics. Vol 9, pp7-19, 2009


Fujii T, Takaya N., Denitrification by the fungus Fusarium oxysporum involves NADH-nitrate reductase. Biosci. Biotechnol. Biochem. Vol 72, pp412-420, 2008


Toyofuku M, Nomura N, Fujii T, Takaya N, Maseda H, Sawada I, Nakajima T, Uchiyama H., Quorum sensing regulates denitrification in Pseudomonas aeruginosa PAO1. J. Bacteriol. Vol 189, pp4969-4972, 2007



Poster Award for Annial Meeting of the Japanse Society of Cellulase, 2012

Poster Award for Annual Meeting of the Japanese Society of Microbial Ecology, 2005